PW01-023 – Ex vivo PBMC cytokine profile in FMF patients
نویسندگان
چکیده
Results Ex vivo cytokine profile The levels of pro-inflammatory cytokines IL-6 and TNF-a were higher in unstimulated and LPS stimulated PBMC supernatants of FMF patients in crises compared to the control group. Concentrations were comparable between FMF patients during and between crises. There was no difference in spontaneous IL1-b and IL-1 a release by PBMCs of FMF patients and controls. However, in response to LPS stimulation, levels of these cytokines were found higher in PBMC supernatants of FMF patients during crises compared to those in remission and to the controls. In contrast, no difference was found in IL1-RA levels between FMF patients and controls in all conditions. Regarding Th1 and Th2 cytokines, IFN-g and IL-4 levels were lower in unstimulated and anti-CD3/CD28 stimulated PBMCs supernatants of FMF patients during and between crises compared to the controls. Moreover, lower levels of those cytokines were detected in culture supernatants of FMF patients during crises compared to those in remission after T cell stimulation. For Th17 cytokines, IL-17 was higher in anti-CD3/ CD28 stimulated PBMC supernatants of FMF patients during crises compared to the control group. After T cell stimulation, PBMCs from FMF patients in remission release more IL-22 than PBMCs from control subjects. Finally, no difference in IL-10 levels was detected between FMF patients and controls.
منابع مشابه
Unstimulated peripheral blood mononuclear cells from patients with the hyper-IgD syndrome produce cytokines capable of potent induction of C-reactive protein and serum amyloid A in Hep3B cells.
The hyper-IgD and periodic fever syndrome (HIDS) and familial Mediterranean fever (FMF) are both characterized by attacks of periodic fever accompanied by acute phase responses that are substantially higher in HIDS than in FMF. To determine whether this difference could be due to differences in production of acute phase protein-inducing mediators, we studied PBMC from HIDS and FMF patients in t...
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Introduction Current recommendations for the treatment of familial Mediterranean fever (FMF) are based largely on the observation of FMF patients receiving colchicine therapy in childhood and young age. The adequate colchicine therapy led to more and more patients survive to that age. In addition, there are national peculiarities of FMF. For example, in Armenia, even before the massive use of c...
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Introduction MEFV is the first identified autoinflammatory gene related to Familial Mediterranean Fever (FMF) disease. We previously the tested the hypothesize of epigenetic involvement in FMF, mainly based on the occurrence of FMF in patients without mutations and decreased MEFV transcripts in leukocyte samples independent from mutations. Our study showed that higher methylation level of MEFV ...
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Introduction Familial Mediterranean fever (FMF) is an inherited autosomal recessive disorder, ethnically restricted and commonly found among individuals of Mediterranean descent, caused by MEditerranean FeVer gene (MEFV) mutations on the chromosome 16. It is the most frequent periodic febrile syndrome among autoinflammatory syndromes. Eighty % of patients with FMF have MEFV mutations, while aro...
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